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Challenge Test

Test the efficacy of your preservative system to ensure microbial safety of your product throughout its shelf life.

 

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Challenge Test

259.00284.90

Objective

The objective of the challenge test, sometimes referred to also as the preservative efficacy test (PET test) is to check the effectiveness of the preservative system of the cosmetic product. Manufacturers of cosmetic products need to ensure that microorganisms introduced during normal product use through the exposure to the environment and from direct contact with the human skin, won’t negatively affect the safety or quality of cosmetic products. This is ensured by performing the challenge test.

Clear

Challenge Test

Challenge test
  • Microogranisms

    Microogranisms
  • Test time

    Test time
  • Sampling times in days

    Sampling times in days
USP 51
284.90 
  • Microogranisms

    Escherichia coli,
    Staphylococcus aureus,
    Pseudomonas aeruginosa,
    Candida albicans,
    Aspergillus brasiliensis
  • Test time

    28 days
  • Sampling times in days

    14 & 28
Japanese Pharmacopoeia
284.90 
  • Microogranisms

    Escherichia coli,
    Staphylococcus aureus,
    Pseudomonas aeruginosa,
    Candida albicans,
    Aspergillus brasiliensis
  • Test time

    28 days
  • Sampling times in days

    7, 14, 21 & 28
European Pharmacopoeia
259.00 
  • Microogranisms

    Staphylococcus aureus,
    Pseudomonas aeruginosa,
    Candida albicans,
    Aspergillus brasiliensis

  • Test time

    28 days
  • Sampling times in days

    0, 2, 7, 14 & 28
ISO 11930:2019
259.00 
  • Microogranisms

    Staphylococcus aureus,
    Pseudomonas aeruginosa,
    Candida albicans,
    Aspergillus brasiliensis

  • Test time

    28 days
  • Sampling times in days

    7, 14 & 28

Challenge Test is required in the following countries:

Non-exhaustive list

Which products do or don’t require it?

Challenge test is required for all cosmetic products with the exception of microbiologically low-risk products, which are the following:

  • Products that don’t contain water/have low water activity
  • Products with a pH below 3 or above 10
  • Products containing more than 20% alcohol
  • Products containing raw materials that can create a hostile environment (strong oxidizing agents, polar organic solvents, oxidizing dyes, aluminium chlorohydrate and related salts, propellant gasses etc.)
  • Products that are filled in containers at more than 65 degrees Celsius
  • Products packaged in pressurized containers, pump dispensers or single-dose units

Which legislations require it?

  • European Union and EEA: consisting of the 27 EU member states as well as Liechtenstein, Norway and Iceland
  • Switzerland
  • United Kingdom
  • Turkey
  • Serbia
  • Gulf Cooperation Council countries: Saudi Arabia, Kuwait, Bahrain, Qatar, United Arab Emirates, Oman
  • ASEAN countries: Indonesia, Thailand, Singapore, Malaysia, Philippines, Vietnam, Cambodia, Brunei, Myanmar, Laos
  • South Africa
  • New Zealand: it’s a standard to provide the challenge test, but it’s not mandatory
  • Argentina
  • Brazil
  • Chile
  • USA and Canada: safety of the product needs to be proven (not prescribed how), challenge test may be one way of doing it

Testing procedure

There is no universal standard for performing the challenge test, but all of the various testing protocols follow the same principles.

Cosmetic product samples are inoculated (artificially contaminated) with different strains of microorganisms, after which the evaluation of the decrease in contamination levels is monitored.

In order for the product to pass the testing, its preservation system has to be sufficiently effective to decrease the level of microorganisms to the allowed, predefined microbial limits. Microorganisms used in challenge testing are usually the following: Pseudomonas aeruginosa, Staphylococcus aeruginosa, Candida albicans and Aspergillus brasiliensis.

There are various standards for performing the challenge test, but there are only small differences between them.
We recommend the ISO 11930:2019 standard, but we can also offer the following:

  • USP 51
  • Japanese Pharmacopoeia
  • European Pharmacopoeia / British Pharmacopoeia
  • ISO 11930:2019

Timeline

Most of the challenge test standards require a 4 week – 28-day test.

We’ll Be There Every Step of the Way
1

Place an order

Find the tests that you need, fill in the necessary data, and press purchase

2

Prepare the samples

You will receive the information about how many samples we need for the requested tests

3

Samples shipping

On checkout you will select a date when our shipping partner will come to pickup the samples

4

Testing

Once we receive the samples, we will confirm the date when the testing will be completed and when you will receive the testing results

5

Testing results

When the tests are completed, we will send you the testing results via email

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